UPLC-QTOF-MS-based lipidomic study of wedelolactone in acute colitis mice induced by dextran sulfate sodium

Inflammatory bowel disease is a relapsing inflammatory seriously endanger human health. Wedelolactone (WED) major active ingredient from Eclipta prostrata (L.) L. and has shown anti-inflammatory effects. However, the mechanism of WED in treating colitis remains unknown. We aimed to investigate mechanisms ulcerative through lipidomic study. Sixty male C57BL/6 mice were exposed DSS induce acute colitis. Disease progression was judged by activity index (DAI) pathological changes colon tissue. An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method performed for plasma lipidomics analyses. Differential metabolites three groups distinguished univariate multivariate analysis. exerted effects representing body weight DAI score. Three identified 20 colon. According pathway analysis, on associated seven pathways. The glycerophospholipid metabolism ether lipid primary findings provide important insight induced perspective.